Spatial Audio Production for Immersive Media Experiences: Perspectives on practice-led approaches to designing immersive audio content

Sound design with the goal of immersion is not new, however, sound design for Immersive Media Experiences (IMEs) utilizing spatial audio can still be considered a relatively new area of practice with less well-defined methods requiring a new and still emerging set of skills and tools. There is, at present, a lack of formal literature around the challenges introduced by this relatively new content form and the tools used to create it, and how these may differ from audio production for traditional media. This article, through the use of semi-structured interviews and an online questionnaire, looks to explore what audio practitioners view as defining features of IMEs, the challenges in creating audio content for IMEs, and how current practices for traditional stereo productions are being adapted for use within 360 interactive soundfields. It also highlights potential direction for future research and technological development and the importance of practitioner involvement in research and development in ensuring future tools and technologies satisfy the current needs

Engineering hemoglobin to enable homogenous PEGylation without modifying protein functionality

In order to infuse hemoglobin into the vasculature as an oxygen therapeutic or blood substitute, it is necessary to increase the size of the molecule to enhance vascular retention. This aim can be achieved by PEGylation. However, using non-specific conjugation methods creates heterogenous mixtures and alters protein function. Site-specific PEGylation at the naturally reactive thiol on human hemoglobin (βCys93) alters hemoglobin oxygen binding affinity and increases its autooxidation rate. In order to avoid this issue, new reactive thiol residues were therefore engineered at sites distant to the heme group and the α/β dimer/dimer interface. The two mutants were βCys93Ala/αAla19Cys and βCys93Ala/βAla13Cys. Gel electrophoresis, size exclusion chromatography and mass spectrometry revealed efficient PEGylation at both αAla19Cys and βAla13Cys, with over 80% of the thiols PEGylated in the case of αAla19Cys. For both mutants there was no significant effect on the oxygen affinity or the cooperativity of oxygen binding. PEGylation at αAla19Cys had the additional benefit of decreasing the rates of autoxidation and heme release, properties that have been considered contributory factors to the adverse clinical side effects exhibited by previous hemoglobin based oxygen carriers. PEGylation at αAla19Cys may therefore be a useful component of future clinical products

Comparison of the oxidative reactivity of recombinant fetal and adult human hemoglobin: implications for the design of hemoglobin-based oxygen carriers.

Hemoglobin based oxygen carriers (HBOCs) have been engineered to replace or augment the oxygen carrying capacity of erythrocytes. However, clinical results have generally been disappointing due, in part due to the intrinsic oxidative toxicity of hemoglobin. The most common HBOC starting material is adult human or bovine hemoglobin. However, it has been suggested that fetal hemoglobin may offer advantages due to decreased oxidative reactivity. Large scale manufacturing of a HBOC will likely ultimately require recombinant sources of human proteins. We therefore directly compared the functional properties and oxidative reactivity of recombinant fetal (rHbF) and recombinant adult (rHbA) hemoglobin. rHbA and rHbF produced similar yields of purified functional protein. No differences were seen in the two proteins in: autoxidation rate; the rate of hydrogen peroxide reaction; NO scavenging dioxygenase activity; and the NO producing nitrite reductase activity. The rHbF protein was: less damaged by low levels of hydrogen peroxide; less damaging when added to human umbilical vein endothelial cells (HUVEC) in the ferric form; and had a slower rate of intrinsic heme loss. The rHbA protein was: more readily reducible by plasma antioxidants such as ascorbate in both the reactive ferryl and ferric states; less readily damaged by lipid peroxides; and less damaging to phosphatidylcholine liposomes. In conclusion in terms of oxidative reactivity there are advantages and disadvantages to the use of recombinant adult or fetal Hb as the basis for an effective HBOC

Semantic audio tools for radio production.

Radio production is a creative pursuit that uses sound to inform, educate and entertain an audience. Radio producers use audio editing tools to visually select, re-arrange and assemble sound recordings into programmes. However, current tools represent audio using waveform visualizations that display limited information about the sound. Semantic audio analysis can be used to extract useful information from audio recordings, including when people are speaking and what they are saying. This thesis investigates how such information can be applied to create semantic audio tools that improve the radio production process. An initial ethnographic study of radio production at the BBC reveals that producers use textual representations and paper transcripts to interact with audio, and waveforms to edit programmes. Based on these findings, three methods for improving radio production are developed and evaluated, which form the primary contribution of this thesis. Audio visualizations can be enhanced by mapping semantic audio features to colour, but this approach had not been formally tested. We show that with an enhanced audio waveform, a typical radio production task can be completed faster, with less effort and with greater accuracy than a normal waveform. Speech recordings can be represented and edited using transcripts, but this approach had not been formally evaluated for radio production. By developing and testing a semantic speech editor, we show that automatically-generated transcripts can be used to semantically edit speech in a professional radio production context, and identify requirements for annotation, collaboration, portability and listening. Finally, we present a novel approach for editing audio on paper that combines semantic speech editing with a digital pen interface. Through a user study with radio producers, we compare the relative benefits of semantic speech editing using paper and screen interfaces. We find that paper is better for simple edits of familiar audio with accurate transcripts

Semantic audio tools for radio production.

Radio production is a creative pursuit that uses sound to inform, educate and entertain an audience. Radio producers use audio editing tools to visually select, re-arrange and assemble sound recordings into programmes. However, current tools represent audio using waveform visualizations that display limited information about the sound. Semantic audio analysis can be used to extract useful information from audio recordings, including when people are speaking and what they are saying. This thesis investigates how such information can be applied to create semantic audio tools that improve the radio production process. An initial ethnographic study of radio production at the BBC reveals that producers use textual representations and paper transcripts to interact with audio, and waveforms to edit programmes. Based on these findings, three methods for improving radio production are developed and evaluated, which form the primary contribution of this thesis. Audio visualizations can be enhanced by mapping semantic audio features to colour, but this approach had not been formally tested. We show that with an enhanced audio waveform, a typical radio production task can be completed faster, with less effort and with greater accuracy than a normal waveform. Speech recordings can be represented and edited using transcripts, but this approach had not been formally evaluated for radio production. By developing and testing a semantic speech editor, we show that automatically-generated transcripts can be used to semantically edit speech in a professional radio production context, and identify requirements for annotation, collaboration, portability and listening. Finally, we present a novel approach for editing audio on paper that combines semantic speech editing with a digital pen interface. Through a user study with radio producers, we compare the relative benefits of semantic speech editing using paper and screen interfaces. We find that paper is better for simple edits of familiar audio with accurate transcripts

Understanding the Patient Experience Through the Power of Film: a Mixed Method Qualitative Research Study

Background For decades film has proved to be a powerful form of communication. Whether produced as entertainment, art or documentary, films have the capacity to inform and move us. Films are a highly attractive teaching instrument and an appropriate teaching method in health education. It is a valuable tool for studying situations most transcendental to human beings such as pain, disease and death. Objectives The objectives were to determine how this helps students engage with their role as health care professionals; to determine how they view the personal experience of illness, disease, disability or death; and to determine how this may impact upon their provision of patient care. Design, Setting and Participants The project was underpinned by the film selection determined by considerate review, intensive scrutiny, contemplation and discourse by the research team. 7 films were selected, ranging from animation; foreign, documentary, biopic and Hollywood drama. Each film was shown discretely, in an acoustic lecture theatre projected onto a large screen to pre-registration student nurses (adult, child and mental health) across each year of study from different cohorts (n = 49). Method A mixed qualitative method approach consisted of audio-recorded 5-minute reactions post film screening; coded questionnaires; and focus group. Findings were drawn from the impact of the films through thematic analysis of data sets and subjective text condensation categorised as: new insights looking through patient eyes; evoking emotion in student nurses; spiritual care; going to the moves to learn about the patient experience; self discovery through films; using films to link theory to practice. Results Deeper learning through film as a powerful medium was identified in meeting the objectives of the study. Integration of film into pre registration curriculum, pedagogy, teaching and learning is recommended. Conclusion The teaching potential of film stems from the visual process linked to human emotion and experience. Its impact has the power to not only help in learning the values that underpin nursing, but also for respecting the patient experience of disease, disability, death and its reality

A Contextual Study of Semantic Speech Editing in Radio Production

Radio production involves editing speech-based audio using tools that represent sound using simple waveforms. Semantic speech editing systems allow users to edit audio using an automatically generated transcript, which has the potential to improve the production workflow. To investigate this, we developed a semantic audio editor based on a pilot study. Through a contextual qualitative study of five professional radio producers at the BBC, we examined the existing radio production process and evaluated our semantic editor by using it to create programmes that were later broadcast. We observed that the participants in our study wrote detailed notes about their recordings and used annotation to mark which parts they wanted to use. They collaborated closely with the presenter of their programme to structure the contents and write narrative elements. Participants reported that they often work away from the office to avoid distractions, and print transcripts so they can work away from screens. They also emphasised that listening is an important part of production, to ensure high sound quality. We found that semantic speech editing with automated speech recognition can be used to improve the radio production workflow, but that annotation, collaboration, portability and listening were not well supported by current semantic speech editing systems. In this paper, we make recommendations on how future semantic speech editing systems can better support the requirements of radio production

Understanding the Patient Experience Through the Power of Film: a Mixed Method Qualitative Research Project

Film is a highly attractive teaching instrument for the study of different terminal diseases, exploring bioethics (Beauchamp and Childress, 2009) and is a preferred medium over traditional lectures (Edmunds, 2013) to provide realistic examples for adult learners. It can tap into ethical issues; facilitate decision-making; and examine underlying issues such as euthanasia; assisted suicide; and professional responsibility. Contrast this with standard means of teaching, such as scenarios- although a useful pedagogic tool, these are limited because students must imagine the clinical scenario. Film can fill that imaginative gap (Volandes, 2007). It can be utilised as an active teaching strategy for a variety of topics in nursing (Edmunds, 2013) providing a unique way to promote active learning in nursing education (Herrman, 2006). The objectives of the study, aim to help pre registration student nurses from each year of study to engage with their role as health care professionals; provide open discussion and debate on how they view the personal experience of illness/disease/disability/death and to reflect on their role and provision of patient care. It is delivered in 3 tiers to provide a range of data for thematic analysis; 1) Film screening followed by a ‘5 minute reaction’ discussion and post screening questionnaire; 2) Pre screening guided activities for reflection and discussion; 3) Focus groups. This project meets identified aims from the UK Professional Standards Framework (UKPSF) by fostering creative and innovative approaches to teaching and learning; facilitating and supporting the design and delivery of continuing education development programmes and activities; and demonstrates professionalism that staff and institutions bring to teaching. Preliminary feedback and themes will be presented